Search results for "Rna folding"

showing 2 items of 2 documents

The effect of genetic robustness on evolvability in digital organisms

2008

Abstract Background Recent work has revealed that many biological systems keep functioning in the face of mutations and therefore can be considered genetically robust. However, several issues related to robustness remain poorly understood, such as its implications for evolvability (the ability to produce adaptive evolutionary innovations). Results Here, we use the Avida digital evolution platform to explore the effects of genetic robustness on evolvability. First, we obtained digital organisms with varying levels of robustness by evolving them under combinations of mutation rates and population sizes previously shown to select for different levels of robustness. Then, we assessed the abilit…

Mutation rateGenetic robustnessDigital organismsGenotypeEvolutionUNESCO::CIENCIAS DE LA VIDA::Otras especialidades de la biologíaPopulationAdaptation BiologicalEvolutionary innovationsBiologyEvolution MolecularQH359-425Computer SimulationSelection GeneticeducationEcology Evolution Behavior and Systematicseducation.field_of_studyGenetic diversityAnalysis of VarianceModels GeneticRobustness (evolution)EvolvabilityGenetic robustness; Digital organisms; Evolutionary innovationsGenetics PopulationPhenotypeEvolutionary biologyMutationTraitRna foldingAvida:CIENCIAS DE LA VIDA::Otras especialidades de la biología [UNESCO]Research ArticleBMC Evolutionary Biology
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Single-Molecule FRET Reveals a Cooperative Effect of Two Methyl Group Modifications in the Folding of Human Mitochondrial tRNALys

2011

Summary Using a combination of advanced RNA synthesis techniques and single molecule spectroscopy, the deconvolution of individual contributions of posttranscriptional modifications to the overall folding and stabilization of human mitochondrial tRNA Lys is described. An unexpected destabilizing effect of two pseudouridines on the native tRNA folding was evidenced. Furthermore, the presence of m 2 G10 alone does not facilitate the folding of tRNA Lys , but a stabilization of the biologically functional cloverleaf shape in conjunction with the principal stabilizing component m 1 A9 exceeds the contribution of m 1 A alone. This constitutes an unprecedented cooperative effect of two nucleotide…

Models MolecularRNA StabilityMolecular Sequence DataClinical BiochemistryContext (language use)BiologyBiochemistryOrganophosphorus CompoundsDrug DiscoveryFluorescence Resonance Energy TransferHumansNucleotideMagnesiumTRNA foldingColoring AgentsMolecular Biologychemistry.chemical_classificationPharmacologyBase SequenceOligonucleotideRNAGeneral MedicineSingle-molecule FRETMitochondriaFolding (chemistry)chemistryBiochemistryTransfer RNABiophysicsNucleic Acid ConformationRNA Transfer LysMolecular MedicinePseudouridineChemistry & Biology
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